Papain

Papain

Introduction:

Papain, a proteolytic enzyme, is widely found in the roots, stems, leaves, and fruits of papaya, and is most abundant in immature milk. The active center of papain contains cysteine, which is a thiol protease. It has the characteristics of high enzyme activity, good thermal stability, natural health and safety, and is therefore widely used in food, medicine, feed, daily chemical, leather and textile industries.

Principle:

The mechanism of papain cleavage peptide bonds includes: Desprotonation of Cys-25 under the action of His-159, and Asn-158 can help the placement of the imidazole ring of His-159, so that deprotonation can occur; then Cys- 25 The nucleophile attacks the carbonyl carbon on the peptide's main chain and covalently connects with it to form an acyl-enzyme intermediate; then the enzyme interacts with a water molecule, deacylation occurs, and the carbonyl end of the peptide chain is

Product features：
1. Temperature range: The effective temperature range of Papain is 10-85 ° C, and the optimal temperature range is 55-66 ° C. The heat resistance is strong.
2. PH value range: The effective pH range is 3.0-9.5, and the optimum pH range is 6.0-7.0.

3. Impact of metal ions on enzyme activity: Papain is susceptible to metal ions such as copper and iron, which reduces the activity. Mg2 + and Ca2 + have a certain degree of inhibition on enzyme activity.

Product properties：

1. Product specifications: The papain solid is a light yellow or white solid powder with an enzyme activity of 30,000u / g; a food-grade solid enzyme activity of 90,000u / g. Liquid Enzyme pH (25 ° C): 3.5-5.0, bulk density: ≤1.25g / ml; fineness of solid enzyme (0.4mm standard sieve pass rate): ≧ 80%.
2. Definition of enzyme activity: Under test conditions (37 ± 0.2 ℃; pH value 7.0), trichloroacetic acid soluble matter released by hydrolysis of casein every minute is at 275nm.When the absorbance at the wavelength is equivalent to that of 1 μg of tyrosine, the amount of enzyme required is one unit of activity.

Instructions：
1. Food industry: The enzyme of papain can be used to hydrolyze the protein of large molecules in food into peptides or amino acids that are easy to absorb.
Widely used in proteolysis of animals and plants such as chicken, pig, cattle, seafood, blood products, soybean, peanut, etc., alcohol clarifier, biscuit loosening agent,Noodle stabilizers, health foods, soy sauce brewing and alcoholic fermentation agents can increase the nutritional value of food and reduce costs.
2. Pharmaceutical industry: drugs containing papain, such as papaya throat tablets, papaya enteric-coated tablets (capsules), have anti-inflammatory, choleretic, analgesic, digestive aid,It can also improve the immunity and other functions. It can also be used to check the surface of red blood cells and blood type identification agents, to treat gynecological diseases, glaucoma, insect bites, etc.
3. Leather textile: hair removal agent made of papain for skin, tanned leather, fine and bright pores; shrinkage prevention of wool.
4. Feed industry: used for feed additives, developing protein sources, facilitating absorption, improving feed utilization, helping livestock digestion and accelerating growth.
5. Daily chemical industry: adding papaya enzyme to cosmetics containing protein and oil, has unique whitening and rejuvenating skin, beauty care, skin stain removal,Promote blood circulation, improve skin and other functions, and improve product quality. It can be made into slimming tea and beauty skin care products.
6. Cosmetics industry: adding papaya enzyme to cosmetics containing protein and oil, has unique whitening and rejuvenation, beauty care, and spot removal Dirt, promote blood circulation, improve skin and other effects, improve product quality. Papain can be made into slimming tea and beauty skin care products.

Packaging and storage：
The solid is packed in food packaging bags, 20kg / bag (can be packed according to customer requirements).

Papain is recommended to store in a cool and dry place, with a relative humidity below 60% and a temperature below 25 °C; the storage period is 12 months, and the storage period in a cool place at 5-15 °C is 24 months.

Packaging and storage of Papain

Solid food packaging bag, 25 kg / barrel.

Papain should be stored in dry, cool and well ventilated places away from the sun, heat.

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