Cellulase

Summary

Cellulases by Trichoderma reesei screening high-yield strain induced by submerged fermentation of purified extract ventilation made. The products are widely used in feed, textile, fuel ethanol, alcohol, herbal extracts, food, fruit juice processing and other industries.

Theory
It refers to cellulases synergistically cellulose β-1,4-glucoside bond the cellulose degradation into short fibers, cellobiose and glucose, a group of enzymes collectively.

Characteristics
1, temperature range: the effective temperature range of Cellulase is 45-75℃, the optimum temperature range of 50-60℃.
2, PH value range: Effective PH range 4.5-7.0, the optimum PH range 6.0-6.5.
3, Effect of metal ions on the enzyme activity: Zn2 +, Mn2 +, Cu2 +, Ca2 + to activate the enzyme, Fe2 +, Fe3 + was strongly inhibited.

Character
1, product specifications: solid cellulase is an off-white powder, 100,000CU/g. Solid enzyme fineness (0.4mm standard sieve through rate): ≥80%.
2, the definition of enzyme activity: 1g solid enzyme powder in 40℃, pH = 4.8 under the conditions of an hour hydrolysis of the sodium carboxymethyl cellulose substrate to produce the equivalent of 1mg of glucose reducing sugar content, a unit of enzyme activity, in u/g expressed.
3, product standards: implementation of industry standards People's Republic of China QB 2583-2003.

Application
1, in the feed complex enzyme, cellulase can improve feed digestibility significantly improve livestock weight gain, milk production and egg production. Improve disease resistance and the ability to adapt the environment. Recommended dosage 0.03-0.1kg/t raw material (5 million CU count).
2, in the textile industry, for the enzyme after silk/linen knits finishing; also suitable for light, fast flowers, dyed back less demanding denim fabric, the fabric has a good softness and drape, permanent anti-pilling, fuzz effect. Add a small amount as a recommended starting point of the experiment (5 million CU count): 0.5-2.0% (owf), PH4.5-5.5, temperature 45-55℃, liquor ratio 1: 10-25, 30-60 minutes .
3, in the production of white wine or cassava alcohol production solid state fermentation, the enzyme can degrade cellulose to sugars, promote the release of cell contents, increase liquor yield, recommended dosage (5 million CU count) 0.15 -0.25kg / t raw material, fermentation or saccharification.
4, the active ingredient in traditional Chinese medicine extraction, the enzyme can destroy the cell walls of dense structure, accelerate the dissolution of pharmaceutical active ingredients, improve the extraction rate of medicinal active ingredient. Recommended amount of 0.05-0.2kg/t raw material (enzyme activity of 5 million CUcount, PH4.5-5.5, temperature 40-50℃, Soak 60-120 minutes. For best results, we recommend and pectinase used in conjunction.
5, cellulase can improve juice yield, can soften the swelling fruit and vegetables and other plant tissue, can promote soybean peeling. It can also be applied to the above sectors. Add a small amount as a recommended starting point for experiments 0.05-0.4L/t pectin (the enzyme activity of 5 million CU units count), maintaining the appropriate temperature and pH conditions for 1-5 hours.

Packaging And Storage
Solid food packaging bags, 25 kg/bucket.

The product of temperature, light, humidity is very sensitive, do as much as possible during transport and storage to avoid exposure, temperature, humidity, kept clean, cool and dry, cold storage can be better.

CERTIFICATE OF ANALYSIS

Packaging and storage of Cellulase

Solid food packaging bag, 25 kg / barrel.

Cellulase should be stored in dry, cool and well ventilated places away from the sun, heat.

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