Bromelain
Summary
Bromelain is a pure natural plant protease obtained by purification, concentration, enzyme immobilization and freeze drying, which extracted from pineapple fruit stem, leaf and skin。It have been widely used in food protein processing and digestion, antiphlogistic medicine, health care products and other industries.
Theory
Bromelain can make polypeptide hydrolyzed into low molecular weight. There are hydrolyzed amide bond and ester effect.
Characteristics
1、Temperature range: The effiective temperature range of bromelain is 40-60℃, the optimum temperature is 50-60℃.
2、The pH range: The effiective pH range is 4.0-8.0, the optimum pH is 4.5-5.5.
3、The effect of metal ions on enzyme activity: It is easy to be influenced by copper, iron and other metal ions, which reduce the activity, and the Mg2+ and Ca2+ have a certain degree of inhibition on the enzyme activity.
Character
1、Product specification:Bromelain is white to light yellow solid,2400GDU/g。
2、Enzyme activity definition:In the determination conditions (37±0.2℃; pH 7.0), the trichloroacetic acid which released by casein has absorbancy on 275nm, when it is fairly with the absorbancy of 1μg tyrosine, the amount of enzyme required is a unit of activity.
3、Product standard:Executive People's Republic of China industry standard GB/T 23527-2009.
Application
1、Temperature range: The effiective temperature range of bromelain is 40-60℃, the optimum temperature is 50-60℃.
2、The pH range: The effiective pH range is 4.0-8.0, the optimum pH is 4.5-5.5.
3、The effect of metal ions on enzyme activity: Bromelain is easy to be influenced by copper, iron and other metal ions, which reduce the activity, and the Mg2+ and Ca2+ have a certain degree of inhibition on the enzyme activity.
Solid for food packaging bags, 25 kg/bucket ( It can be packaged according to customer requirements).
Stored in a cool dry place, and the relative humidity less than 60%, and the temperature is below 25℃; The
storage life is 12months, if stored on a 5~15℃, cool and dry environnment, it can be stored for 24months, and it is best to stay away from the smell of stored material.
CERTIFICATE OF ANALYSIS
Product Name
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Bromelain
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|
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Other Name
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NA
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Country of Origin
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China
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Strain
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pineapple
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Manufacture Date
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OCT 19, 2018
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Batch Number
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BR18101901
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Expiration Date
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OCT 18, 2020
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Package
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25 kg/bucket
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Quantity
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350 kg
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PROTOCOL
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SPECIFICATIONS
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RESULTS
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METHOD
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PHYSICAL & CHEMICAL ANALYSIS
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Description
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yellowish-brown or brown powder
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Complies
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Visual
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Odor & Taste
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Characteristic odor and taste
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Complies
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Taste
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Moisture
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NMT 7%
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4.3%
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Moisture Analyzer
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IDENTIFICATION
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Identifiable Activity
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Positive for Bromelain Activity
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Complies
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In-house
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ACTIVITY
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Bromelain activity
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NLT 2,800GDU/g
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2,809
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In-house
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MICROBIOLOGICAL
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Total bacteria count
Coliform bacteria (CFU/g)
Molds and Yeasts
E.Coli
Salmonella
Staphylococcus aureus
Pseudomonas aeruginosa
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NMT 3,000 CFU/g
NMT 30 CFU/g
NMT 100 CFU/g
Absent
Absent
Absent
Absent
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Complies
Complies
Complies
Complies
Complies
Complies
Complies
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FDA BAM Online Ch.3
FDA BAM Online Ch.4
FDA BAM Online Ch. 2
FDA BAM Online Ch.4
FDA BAM Online Ch.5
FDA BAM Online Ch.12
AOAC
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HEAVY METALS
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Lead
Mercury
Cadmium
Arsenic
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NMT 3 ppm
NMT 0.1 ppm
NMT 1 ppm
NMT 1 ppm
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<3 ppm
<0.1 ppm
<1 ppm
Complies
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USP <231>
USP <231>
USP <231>
USP <231>
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Storage: Store in cool and dry place protected from light, keep drum close when not in use.
Conclusion: Conforms to IU Standards.
Shelf Life: Shelf life of bromelain under prescribed storage conditions and air-tight packing will be 2 years.
The product COMPLIES with the above said specifications
Approved by RICKY H. ZHU
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Packaging and storage
Solid food packaging bag, 25 kg / barrel.
Bromelain should be stored in dry, cool and well ventilated places away from the sun, heat.
Payment :T/T, Paypal, Western Union, Alibaba Trade Assurance, VISA, MasterCard,e-Checking, Pay Later, LC and etc.
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