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Bromelain
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Bromelain

Bromelain is a pure natural plant protease obtained by purification, concentration, enzyme immobilization and freeze drying.

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Product Description


Bromelain


Summary

Bromelain is a pure natural plant protease obtained by purification, concentration, enzyme immobilization and freeze drying, which extracted from pineapple fruit stem, leaf and skin。It have been widely used in food protein processing and digestion, antiphlogistic medicine, health care products and other industries.


Theory
Bromelain can make polypeptide hydrolyzed into low molecular weight. There are hydrolyzed amide bond and ester effect.

Characteristics
1、Temperature range: The effiective temperature range of bromelain is 40-60℃, the optimum temperature is 50-60℃.
2、The pH range: The effiective pH range is 4.0-8.0, the optimum pH is 4.5-5.5.
3、The effect of metal ions on enzyme activity: It is easy to be influenced by copper, iron and other metal ions, which reduce the activity, and the Mg2+ and Ca2+ have a certain degree of inhibition on the enzyme activity.

Character
1、Product specification:Bromelain is white to light yellow solid,2400GDU/g。
2、Enzyme activity definition:In the determination conditions (37±0.2℃; pH 7.0), the trichloroacetic acid which released by casein has absorbancy on 275nm, when it is fairly with the absorbancy of 1μg tyrosine, the amount of enzyme required is a unit of activity.
3、Product standard:Executive People's Republic of China industry standard GB/T 23527-2009.

Application
1、Temperature range: The effiective temperature range of bromelain is 40-60℃, the optimum temperature is 50-60℃.
2、The pH range: The effiective pH range is 4.0-8.0, the optimum pH is 4.5-5.5.
3、The effect of metal ions on enzyme activity: Bromelain is easy to be influenced by copper, iron and other metal ions, which reduce the activity, and the Mg2+ and Ca2+ have a certain degree of inhibition on the enzyme activity.

Solid for food packaging bags, 25 kg/bucket ( It can be packaged according to customer requirements).
Stored in a cool dry place, and the relative humidity less than 60%, and the temperature is below 25℃; The

storage life is 12months, if stored on a 5~15℃, cool and dry environnment, it can be stored for 24months, and it is best to stay away from the smell of stored material.


CERTIFICATE OF ANALYSIS


Product Name Bromelain    
Other Name NA Country of Origin China
Strain pineapple Manufacture Date OCT 19, 2018
Batch Number BR18101901 Expiration Date OCT 18, 2020
Package 25 kg/bucket Quantity 350 kg
PROTOCOL SPECIFICATIONS RESULTS METHOD
PHYSICAL & CHEMICAL ANALYSIS
Description yellowish-brown or brown powder Complies Visual
Odor & Taste Characteristic odor and taste Complies Taste
Moisture NMT 7% 4.3% Moisture Analyzer
IDENTIFICATION
Identifiable Activity Positive for Bromelain Activity Complies In-house
ACTIVITY
Bromelain activity NLT 2,800GDU/g 2,809 In-house
MICROBIOLOGICAL
Total bacteria count
Coliform bacteria (CFU/g)
Molds and Yeasts
E.Coli
Salmonella
Staphylococcus aureus
Pseudomonas aeruginosa
NMT 3,000 CFU/g
NMT 30 CFU/g
NMT 100 CFU/g
Absent
Absent
Absent
Absent
Complies
Complies
Complies
Complies
Complies
Complies
Complies
FDA BAM Online Ch.3
FDA BAM Online Ch.4
FDA BAM Online Ch. 2
FDA BAM Online Ch.4
FDA BAM Online Ch.5
FDA BAM Online Ch.12
AOAC
HEAVY METALS
Lead
Mercury
Cadmium
Arsenic
NMT 3 ppm
NMT 0.1 ppm
NMT 1 ppm
NMT 1 ppm
<3 ppm
<0.1 ppm
<1 ppm
Complies
USP <231>
USP <231>
USP <231>
USP <231>
Storage: Store in cool and dry place protected from light, keep drum close when not in use.
Conclusion: Conforms to IU Standards.
Shelf Life: Shelf life of bromelain under prescribed storage conditions and air-tight packing will be 2 years.
The product COMPLIES with the above said specifications
Approved by RICKY H. ZHU


Packaging and storage

Solid food packaging bag, 25 kg / barrel.


Bromelain should be stored in dry, cool and well ventilated places away from the sun, heat.

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